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KMID : 0363320130340020178
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Journal of Korean Oriental Internal Medicine
2013 Volume.34 No. 2 p.178 ~ p.191
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Protective Effects of Ukgan-san in CoCl2-induced Cell Death of C6 Glial Cells
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Cho Mun-Young
Shin Yong-Jeen
Ha Ye-Jin
Woo Chan
Kim Ta-Jung
You Ju-Yeon
Choi Yong-Seok
Choi Jung-Hoon
Shin Sun-Ho
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Abstract
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Objectives : In this study, we made an effort to investigate the protective mechanism of Ukgan-san (UGS) extracts on
hypoxia-induced C6 glial cell death.
Methods : The cell viability was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MMT) assay
and cell morphological changes were analysed with microscope after staining with crystal violet (CV). Reactive oxygen species (ROS) formation was assessed by flow cytometer after staining with 2'7'-dichlorofluorescein diacetate (DCF-DA). We also analyzed expression of hypoxia-inducible factor-1 alpha (HIF-1α) and p53, processing of procaspase-3 and procyclic acidic repetitive protein (PARP) by western blot method.
Results : We estimated the elevated cell viability by UGS extract on CoCl2-induced C6 glial cells. UGS attenuated
CoCl2-induced ROS formation in C6 glial cells and also showed a protective activity compared to antioxidants and exhibited abrogation of LDH-released by CoCl2. UGS suppressed the typical apoptotic cell death markers, caspase-3 and PARP activation. UGS inhibited CoCl2-induced HIF-1α expression which is known as a major regulator for hypoxia-induced cell death, and suppressed p53 expression.
Conclusions : These results suggest that UGS extract contains protective constituents for hypoxia-induced C6 glial cell
death.
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KEYWORD
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CoCl2, C6 glial cell, protective effect, Ukgan-san (Yigan-san)
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